Barlow was recalled from Triple-A Omaha for Game 2 of Saturday's doubleheader against the White Sox.
Barlow, who has served as the Royals' 26th man for two doubleheaders already this season, will be active for Game 2 on Saturday. The 25-year-old returned to the minors immediately both times and has yet to make his major-league debut. Barlow has struck out 13 and allowed one run on nine hits and seven walks over 12 innings with Omaha this season.