Culpepper was removed from Tuesday's game against the Yankees with left hamstring tightness, Aaron Gleeman of AaronGleeman.com reports.
Culpepper seemed to be grabbing at his leg after grounding into a double play in the sixth inning, and the Twins took him out of the game at the start of the seventh. The severity of his injury is unclear, but Ryan Kreidler could benefit from extra starts if Culpepper is unable to play.